Quantitative Detection of Bifidobacterium longum Strains in Feces Using Strain-Specific Primers
نویسندگان
چکیده
منابع مشابه
Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers
For the first time in Iran 40 strains of Bifidobacterium were isolated from feces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as Bifidobacterium longum, 10 as Bifidobacterium bifidum and one as Bifidobacterium catenolatum. In order to validate these results and also to identify other isolates that had not been identified by phenotypic tests, two methods of PCR wi...
متن کاملDifferentiation of Bifidobacterium longum subspecies longum and infantis by quantitative PCR using functional gene targets
BACKGROUND Members of the genus Bifidobacterium are abundant in the feces of babies during the exclusively-milk-diet period of life. Bifidobacterium longum is reported to be a common member of the infant fecal microbiota. However, B. longum is composed of three subspecies, two of which are represented in the bowel microbiota (B. longum subsp. longum; B. longum subsp. infantis). B. longum subspe...
متن کاملBioaccessible Antioxidants in Milk Fermented by Bifidobacterium longum subsp. longum Strains
Bifidobacterium longum subsp. longum is among the dominant species of the human gastrointestinal microbiota and could thus have potential as probiotics. New targets such as antioxidant properties have interest for beneficial effects on health. The objective of this study was to evaluate the bioaccessibility of antioxidants in milk fermented by selected B. longum subsp. longum strains during in ...
متن کاملDraft Genome Sequence of the Probiotic Bifidobacterium longum subsp. longum Strain MC-42
Here, we report the draft genome sequence of Bifidobacterium longum subsp. longum strain MC-42 isolated from the feces of a healthy infant, and which was used in the commercially available probiotic product Biovestin.
متن کاملبررسی فراوانی گونه‌های بیفیدوباکتریوم جداشده از شیر مادر و مدفوع نوزادان مناطق روستایی استان مرکزی در سال 1394
Background: Intestinal colonization of the newborn is essential for establishment, maturation and maintenance of the gut mucosal barrier. The greatest difference between the microbiota of breast milk and Infant formula infants feeding is the numbers and species composition of Bifidobacteria. In this study, we tried to identify the native Bifidobacterium isolates obtained from the human’s breast...
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ژورنال
عنوان ژورنال: Microorganisms
سال: 2021
ISSN: 2076-2607
DOI: 10.3390/microorganisms9061159